What the barcode file format?

Open echovv opened this issue 2 years ago • 1 comments

Hi! I want to split sample from fastq. But when I run seqtk_demultiplex command, I get the reminder-"barcode file format error!". So I want to know what the correct format. And It seems the tutor link can't not be opened! http://www.biostack.org/?p=428. Here I provide a link（链接：https://pan.baidu.com/s/14cASnBFtEtRlm6-mYJMkrQ 提取码：0909 --来自百度网盘超级会员V4的分享） including all my data I run this time. Thanks your attention. Looking forward to your reply!

Mar 15 '24 05:03 echovv

You can you atlas-utils demux (https://github.com/jameslz/atlas-utils) with the command:

atlas-utils  demultiplex -b barcode.txt -1 2023-11-7_raw_1.fq.gz -2 2023-11-7_raw_2.fq.gz  -l  9 -d  BARCODE

and any blank lines in barcode.txt file should be removed.

Mar 15 '24 08:03 jameslz