Help with single library with one replicate

[Biomaagi]

Hi,

How would one run SCENT in a situation that has only one sample and batch available? As of now, I have tried to omit these two covariates from the SCENT model, but this seems to cause some peculiar outputs with the bootstrap p-value being significantly poorer compared to the one from Poisson regression. Additionally, the bootstrap p-values seem to come out in sequences, where the same p-val is repeated for hundreds or thousands of gene-peak pairs. Is this the expected behaviour? From what I gather, wouldn't you expect only the lowest p-value to repeat when the calculation hits its cap?

Thank you in advance!