hallamlab
treesapp assign error
Hello!
I am a master student in evolutionary biology trying to run treesapp assign on a fasta file containing 11 a.a sequences for the mitochondrial gene ATPase6 (and have tried with other mitochondrial genes but no success), and the following error occures:
Arguments used: -i ATPase6_diploids.faa --trim_align --num_procs 8 -o /home/cayna/miniconda3/envs/treesapp_cenv/mydata/ATPase6 TreeSAPP will analyze the 11 sequences found in input. Parsing HMMER domain tables for high-quality matches... done. WARNING: No alignments found! TreeSAPP is exiting now.
I really don't understant why this is happening, although the test provided - treesapp assign -i TreeSAPP/tests/test_data/marker_test_suite.faa -m prot --trim_align -o assign_test -t McrA,DsrAB - worked just fine.
I have tried to create a refpkg using the same fasta file containing 11 a.a sequences for the mitochondrial gene ATPase6, to see if it would fix anything but i wasn't successfull because it kept removing all sequences except one because it considered them all equal.
I really don't know how to proceed... It's my first time using TreeSAPP so i would immensly appreciate your help!
To Reproduce Steps to reproduce the behavior:
- Run the command treesapp assign -i ATPase6_diploids.faa --trim_align --num_procs 8 -o ~/miniconda3/envs/treesapp_cenv/mydata/ATPase6
- See error:
Arguments used: -i ATPase6_diploids.faa --trim_align --num_procs 8 -o /home/cayna/miniconda3/envs/treesapp_cenv/mydata/ATPase6 TreeSAPP will analyze the 11 sequences found in input. Parsing HMMER domain tables for high-quality matches... done. WARNING: No alignments found! TreeSAPP is exiting now.
- See file : TreeSAPP_classify_log.txt
System information (please complete the following information):
- OS: Windows subsystem for Linux - Ubuntu 18.04.5
- Python version 3.7.12
- TreeSAPP Version 0.11.4
