meme problen:Failed to create PNG file;make_cut_matrix mistake
hi,fulong,"I am facing an issue with DREME3-py where I cannot generate PNG files properly, while other files can be generated successfully. Additionally, my ATACtk is not running as expected."
Starting meme: meme random1000/MEME_PRDM9_shuf/seqs-centered -oc random1000/MEME_PRDM9_shuf/meme_out -mod zoops -nmotifs 10 -minw 6 -maxw 30 -bfile random1000/MEME_PRDM9_shuf/background -dna -revcomp -nostatus meme ran successfully in 2570.817556 seconds Starting dreme-py3: dreme-py3 -verbosity 1 -oc random1000/MEME_PRDM9_shuf/dreme_out -png -dna -p random1000/MEME_PRDM9_shuf/seqs-centered -n random1000/MEME_PRDM9_shuf/seqs-shuffled -m 10 Failed to create PNG file. Have you got ghostscript installed? Failed to create PNG file. Have you got ghostscript installed? Failed to create PNG file. Have you got ghostscript installed? Failed to create PNG file. Have you got ghostscript installed? Failed to create PNG file. Have you got ghostscript installed? Failed to create PNG file. Have you got ghostscript installed?
[info] Doing fimo2.DREME-10.AHCTAAAC...
make_cut_matrix -v -b '(25-150 1)' -d -o 0 -r 100 -p 1 -f 3 -F 4 -F 8 -q 0 /media/dell/data2/chenzhiyi/23.11.3_wangbingyi/output/BN-2/aligned/dup.marked.120bp/BN-2.bam /media/dell/data2/chenzhiyi/23.11.3_wangbingyi/output/BN-2/peakcalling/macs2.narrow/BN-2_summits.bed > fimo.cuts.freq.txt
Traceback (most recent call last):
File "/home/chenzhiyi/.local/bin/make_cut_matrix", line 277, in
"For the issue of being unable to create PNG files, I found that changing the output format of DREME3 from png to eps resolved the problem. For the problem of not being able to generate fimo.cuts.freq.txt, when I use ATACtk installed directly from the official website without applying any patches, the program runs successfully."