Error no cell types passed weight threshold on full mode

[Yijia-Jiang]

Hello, I received the following error when running RCTD. Could you let me know what might have caused this error? Thank you very much. The code I used is:

myRCTD <- run.RCTD(myRCTD, doublet_mode = 'multi')

The error message shows:

process_bead_multi: no cell types passed weight threshold on full mode. Please check that enough counts are present for each pixel

[dmcable]

This means that when full mode was run on RCTD, for at least one pixel, no cell type earned a weight of at least initial_weight_thresh = 0.01.

To debug this issue, you should search for such a pixel after running RCTD in full mode. One likely culprit is an issue with the total UMI counts variable.