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Mismatch in read quality between input and simulated reads

Open ezherman opened this issue 6 months ago • 2 comments

Hi,

We have been using NanoSim v3.2.3, and comparing the read qualities in input and simulated reads.

Input

We are characterising on approx 300k reads with the following quality distribution:

Image

Output

We are simulating 100k reads. The simulated reads have a narrower quality score distribution, without many reads below Q20 or above Q30:

Image

Questions

Is this expected behaviour? And are there parameters we could tweak?

Thanks!

ezherman avatar Jul 17 '25 13:07 ezherman