NanoSim
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Mismatch in read quality between input and simulated reads
Hi,
We have been using NanoSim v3.2.3, and comparing the read qualities in input and simulated reads.
Input
We are characterising on approx 300k reads with the following quality distribution:
Output
We are simulating 100k reads. The simulated reads have a narrower quality score distribution, without many reads below Q20 or above Q30:
Questions
Is this expected behaviour? And are there parameters we could tweak?
Thanks!