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pyscenic warning: less than 80% of the genes in xxx could be mapped to ...

Open Beki-seq opened this issue 2 years ago • 3 comments

Describe the bug Hello pyscenic team,

I conducted pyscenic analysis, and I use "hg19-500bp-upstream-10species.mc9nr.genes_vs_motifs.rankings.feather". All other things works fine, expect the log file documented that less than 80% of the genes in xxx could be mapped to hg19-500bp-upstream-10species.mc9nr.genes_vs_motifs.rankings.feather. The model is skipped. I also tried to use "hg19-tss-centered-10kb-7species.mc9nr.genes_vs_motifs.rankings.feather" But the log file still noted similar error.

For my previous analysis, I conduced in R with Seurat, and when I run the cell ranger I use hg19. I checked the CSV file I use at the beginning, and the first line is sample 1, sample 2 and etc. The first row is the gene name, thus I believe my CSV file is in correct formate.

Steps to reproduce the behavior

  1. Error encountered: The error noticed after complete running the pyscenic code.

Please complete the following information: Following is the code how I install pyscenic. $ module load miniconda3/38_4.9.2 $ eval “$(/risapps/rhel7/miniconda3/38_4.9.2/bin/conda shell.bash hook)” $ conda create -p ${HOME}/.conda/envs/pyscenic-0.12.1 python=3.10.9 $ conda activate pyscenic-0.12.1 $ conda install pyarrow $ wget

Beki-seq avatar Mar 22 '23 21:03 Beki-seq

This warning less than 80% of the genes in xxx could be mapped to ... mean the gene names used in the expression matrix, do not match the gene names used in the feather file. Make sure your gene names of your expression matrix match the ones in the feather file (you might want the hg38 versions of the databases).

ghuls avatar Jun 13 '23 10:06 ghuls

@ghuls

How do we change the threshold value of 80% to lets say 50% ?

GeneVector5 avatar Jul 21 '23 22:07 GeneVector5