Try resazurin with shaking/mechanical stress instead of temperature stress

[AHuffmyer]

[AHuffmyer]

Procedure:

1. Gather 10-20 oysters and have half as control (no stress) and spinning (mechanical stress)
2. Spin the treatment oysters in a salad spinner for 10 minutes, leave the control on the counter
3. Add to resazurin as you normally do for other trials and leave all oysters (control and treated) on the counter. This trial will be run at control temperature.
4. Take a T0 sample and read using fluorescence on the plate reader
5. Take a sample and read using fluorescence every hour for 3-4 hours.
6. Analyze data to see if there is a difference in fluorescence between oysters that were mechanically stress and those that were not.

[AHuffmyer]

@Noah-Oz please update your notebook posts and attach the raw/Excel data files here!

[AHuffmyer]

Trials show higher metabolic rates in mechanically stressed oysters (stressed before each time point measurement) than control oysters. Worth exploring in future trials!

Mechanical Stress.xlsx