resources ID oyster diploid/triploid families

Some pics from PW...

There were 4 bags in one upweller. we color coded the four bags...

@AHuffmyer might have more useful info to add

Blues were clearly biggest and had the largest numbers

Jul 29 '23 00:07 sr320

No notes to add - sizes and shell colors were pretty variable in the other bags, so there were no clear phenotype differences.

Jul 31 '23 16:07 AHuffmyer

I took a few photos of the upwellers when I placed the bags in there. Here is a presentation with the pictures and my best guesses as to which families are in which. As memory also serves, there were not very many F5 3n, but they were big!

Aug 02 '23 21:08 mattgeorgephd

we only dealt with outside tanks -- did not know any are / were inside...

note F14 (2n) were outside

We have now know cohorts (bags) as

Orange Pink Blue Yellow

thus

Yellow = F14 (2n)

My take is we should have 8 bags but only have 4? @mattgeorgephd sorry but missed your best guesses?

Aug 16 '23 18:08 sr320

I did locate four more bags

Thus to date it is likely

Orange = ? Pink = ? Blue = ? Yellow = F14 (2n) Dog [Putnam hobo] = F5 (2n)? Cat [very big, few] = F5 (3n) Bird [Tank 3 hobo] = F13 (either 2n or 3n) Toad = F14 (3n)

Aug 19 '23 00:08 sr320

Do we have updates to this list or do unknowns remain unknown?

Apr 04 '24 18:04 AHuffmyer

No, we really need to coordinate with Pacific hybrid to determinative if they can id with cytometer (dip vs trip)

On Thu, Apr 4, 2024 at 11:15 AM Ariana S Huffmyer @.***> wrote:

Do we have updates to this list or do unknowns remain unknown?

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Apr 04 '24 22:04 sr320

Is that something we can do or would we need to send them animals?

Apr 04 '24 23:04 AHuffmyer

Benoit at Quilcene Hatchery also has a ploidy analyzer

Apr 08 '24 04:04 mattgeorgephd

Alexa (PH) has agreed to do this. The next step is for me to somehow get the oysters to her in Manchester.

Apr 21 '24 19:04 sr320

I can take oysters to Manchester next week - I could pick up on a Point Whitney day and deliver to Manchester.

Apr 21 '24 22:04 AHuffmyer

Delivered to Manchester on 5/7; Alexa analyzing on 4/8

May 08 '24 14:05 AHuffmyer

Results from analyzed cohorts here: https://ahuffmyer.github.io/ASH_Putnam_Lab_Notebook/Oyster-ploidy-results/

Need to confirm other families not detailed in this post

May 29 '24 04:05 AHuffmyer

before

Orange = ? Pink = ? Blue = ? Yellow = F14 (2n) Dog [Putnam hobo] = F5 (2n)? Cat [very big, few] = F5 (3n) Bird [Tank 3 hobo] = F13 (either 2n or 3n) Toad = F14 (3n)

AFTER analyzes

Orange = ? triploid Pink = ? Blue = ? Tetraploid Yellow = F14 (2n) diploid Dog [Putnam hobo] = F5 (2n)? triploid Cat [very big, few] = F5 (3n) Bird [Tank 3 hobo] = F13 (either 2n or 3n) triploid Toad = F14 (3n)

Jun 10 '24 21:06 sr320

I believe we have done all the identification we can do up to this point, correct? I'll close if so.

Sep 13 '24 22:09 AHuffmyer

@sr320 the Pink family was used in the lifestage carryover project and it would be good to ID if diploid or triploid. I have samples in ethanol for DNA. How would you like to proceed with identifying these? One option would be a microsat method like this one: https://pmc.ncbi.nlm.nih.gov/articles/PMC4093491/

Dec 21 '24 20:12 AHuffmyer