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verified publisher icon RajLabMSSM

Incorporating POLYFUN

Open JonnyBaseball opened this issue 3 years ago • 3 comments

1. Bug description

PolyFun is not installed. Reinstall echofinemap with PolyFun? This will automatically restart R. (y/n): Error in echofinemap::POLYFUN_install() : PolyFun is not installed.

Awesome package! Also thanks in advance for any help you can give me!

Expected behaviour

Your example echodata::BCST results included POLYFUN_SUSIE results.

2. Reproducible example

results <- echolocatoR::finemap_loci(

  • fullSS_path = cf.long.munged,
  • topSNPs = topSNPsmPth,
  • loci = c("ABHD17C","AFF3","ATXN","C20orf112","C6orf106"),
  • LD_reference = "1KGphase3",
  • #dataset_name = "cf.longevity",
  • fullSS_genome_build = "hg19",
  • bp_distance = 100000,
  • finemap_methods = c("ABF","SUSIE","FINEMAP","POLYFUN_FINEMAP","POLYFUN_SUSIE","PAINTOR","COJO_conditional","COJO_joint","COJO_stepwise"),
  • munged = TRUE)

Console output

PolyFun is not installed. Reinstall echofinemap with PolyFun? This will automatically restart R. (y/n): Error in echofinemap::POLYFUN_install() : PolyFun is not installed.

Data

As set forth in the next comment, I tried to access Polyfun using your example data. There I also responded 'y' to option to install Polyfun.

If it would help to see my data, I can certainly upload a small sample. Note that there was no problem when I cut out all but "ABF" (not relevant since not binary), "SUSIE", and "FINEMAP". I used a GWAS generated using GSEM (with 6.7 million snps, munged down using MungeSumstats to 3.7 million due to multiallelic snps).

(If possible, upload a small sample of your data so that we can reproduce the bug on our end. If that's not possible, please at least include a screenshot of your data and other relevant details.)

3. Session info

(Add output of the R function utils::sessionInfo() below. This helps us assess version/OS conflicts which could be causing bugs.)

> utils::sessionInfo()
R version 4.2.0 (2022-04-22)
Platform: x86_64-apple-darwin17.0 (64-bit)
Running under: macOS Catalina 10.15.7

Matrix products: default
BLAS:   /Library/Frameworks/R.framework/Versions/4.2/Resources/lib/libRblas.0.dylib
LAPACK: /Library/Frameworks/R.framework/Versions/4.2/Resources/lib/libRlapack.dylib

locale:
[1] en_US.UTF-8/en_US.UTF-8/en_US.UTF-8/C/en_US.UTF-8/en_US.UTF-8

attached base packages:
[1] stats4    stats     graphics  grDevices utils     datasets  methods   base     

other attached packages:
[1] snpStats_1.48.0     Matrix_1.5-3        survival_3.4-0      readr_2.1.3         GenomeInfoDb_1.34.3 IRanges_2.32.0      S4Vectors_0.36.0   
[8] BiocGenerics_0.44.0 echolocatoR_2.0.3  

loaded via a namespace (and not attached):
  [1] rappdirs_0.3.3                              rtracklayer_1.58.0                          GGally_2.1.2                               
  [4] R.methodsS3_1.8.2                           ragg_1.2.4                                  tidyr_1.2.1                                
  [7] echoLD_0.99.8                               ggplot2_3.4.0                               bit64_4.0.5                                
 [10] knitr_1.41                                  irlba_2.3.5.1                               DelayedArray_0.24.0                        
 [13] R.utils_2.12.2                              data.table_1.14.6                           rpart_4.1.19                               
 [16] KEGGREST_1.38.0                             RCurl_1.98-1.9                              AnnotationFilter_1.22.0                    
 [19] generics_0.1.3                              GenomicFeatures_1.50.2                      RSQLite_2.2.19                             
 [22] proxy_0.4-27                                bit_4.0.5                                   tzdb_0.3.0                                 
 [25] xml2_1.3.3                                  SummarizedExperiment_1.28.0                 assertthat_0.2.1                           
 [28] viridis_0.6.2                               gargle_1.2.1                                xfun_0.35                                  
 [31] hms_1.1.2                                   fansi_1.0.3                                 restfulr_0.0.15                            
 [34] progress_1.2.2                              dbplyr_2.2.1                                readxl_1.4.1                               
 [37] Rgraphviz_2.42.0                            igraph_1.3.5                                DBI_1.1.3                                  
 [40] htmlwidgets_1.5.4                           reshape_0.8.9                               downloadR_0.99.5                           
 [43] purrr_0.3.5                                 ellipsis_0.3.2                              dplyr_1.0.10                               
 [46] ggnewscale_0.4.8                            backports_1.4.1                             biomaRt_2.54.0                             
 [49] deldir_1.0-6                                MatrixGenerics_1.10.0                       MungeSumstats_1.7.2                        
 [52] vctrs_0.5.1                                 Biobase_2.58.0                              ensembldb_2.22.0                           
 [55] cachem_1.0.6                                withr_2.5.0                                 BSgenome_1.66.1                            
 [58] checkmate_2.1.0                             vroom_1.6.0                                 GenomicAlignments_1.34.0                   
 [61] prettyunits_1.1.1                           cluster_2.1.4                               ape_5.6-2                                  
 [64] dir.expiry_1.6.0                            lazyeval_0.2.2                              crayon_1.5.2                               
 [67] basilisk.utils_1.10.0                       crul_1.3                                    pkgconfig_2.0.3                            
 [70] labeling_0.4.2                              nlme_3.1-160                                ProtGenerics_1.30.0                        
 [73] XGR_1.1.8                                   nnet_7.3-18                                 pals_1.7                                   
 [76] rlang_1.0.6                                 lifecycle_1.0.3                             filelock_1.0.2                             
 [79] httpcode_0.3.0                              BiocFileCache_2.6.0                         echotabix_0.99.8                           
 [82] dichromat_2.0-0.1                           cellranger_1.1.0                            coloc_5.1.0.1                              
 [85] matrixStats_0.63.0                          graph_1.76.0                                osfr_0.2.9                                 
 [88] boot_1.3-28.1                               base64enc_0.1-3                             png_0.1-8                                  
 [91] viridisLite_0.4.1                           rjson_0.2.21                                rootSolve_1.8.2.3                          
 [94] bitops_1.0-7                                R.oo_1.25.0                                 ggnetwork_0.5.10                           
 [97] Biostrings_2.66.0                           blob_1.2.3                                  mixsqp_0.3-48                              
[100] stringr_1.4.1                               echoplot_0.99.6                             dnet_1.1.7                                 
[103] jpeg_0.1-9                                  echodata_0.99.16                            BSgenome.Hsapiens.1000genomes.hs37d5_0.99.1
[106] scales_1.2.1                                memoise_2.0.1                               magrittr_2.0.3                             
[109] plyr_1.8.8                                  hexbin_1.28.2                               zlibbioc_1.44.0                            
[112] compiler_4.2.0                              echoconda_0.99.8                            BiocIO_1.8.0                               
[115] RColorBrewer_1.1-3                          catalogueR_1.0.0                            EnsDb.Hsapiens.v75_2.99.0                  
[118] Rsamtools_2.14.0                            cli_3.4.1                                   XVector_0.38.0                             
[121] echoannot_0.99.10                           patchwork_1.1.2                             htmlTable_2.4.1                            
[124] Formula_1.2-4                               MASS_7.3-58.1                               tidyselect_1.2.0                           
[127] stringi_1.7.8                               textshaping_0.3.6                           yaml_2.3.6                                 
[130] supraHex_1.36.0                             latticeExtra_0.6-30                         ggrepel_0.9.2                              
[133] grid_4.2.0                                  VariantAnnotation_1.44.0                    tools_4.2.0                                
[136] lmom_2.9                                    parallel_4.2.0                              rstudioapi_0.14                            
[139] foreign_0.8-83                              piggyback_0.1.4                             gridExtra_2.3                              
[142] gld_2.6.6                                   farver_2.1.1                                RcppZiggurat_0.1.6                         
[145] digest_0.6.30                               BiocManager_1.30.19                         Rcpp_1.0.9                                 
[148] GenomicRanges_1.50.1                        OrganismDbi_1.40.0                          httr_1.4.4                                 
[151] AnnotationDbi_1.60.0                        RCircos_1.2.2                               ggbio_1.46.0                               
[154] biovizBase_1.46.0                           colorspace_2.0-3                            XML_3.99-0.12                              
[157] fs_1.5.2                                    reticulate_1.26                             splines_4.2.0                              
[160] RBGL_1.74.0                                 expm_0.999-6                                echofinemap_0.99.4                         
[163] basilisk_1.10.2                             Exact_3.2                                   mapproj_1.2.9                              
[166] SNPlocs.Hsapiens.dbSNP155.GRCh37_0.99.22    systemfonts_1.0.4                           jsonlite_1.8.3                             
[169] Rfast_2.0.6                                 susieR_0.12.27                              R6_2.5.1                                   
[172] Hmisc_4.7-2                                 pillar_1.8.1                                htmltools_0.5.3                            
[175] glue_1.6.2                                  fastmap_1.1.0                               DT_0.26                                    
[178] BiocParallel_1.32.3                         class_7.3-20                                codetools_0.2-18                           
[181] maps_3.4.1                                  mvtnorm_1.1-3                               utf8_1.2.2                                 
[184] lattice_0.20-45                             tibble_3.1.8                                curl_4.3.3                                 
[187] DescTools_0.99.47                           zip_2.2.2                                   openxlsx_4.2.5.1                           
[190] interp_1.1-3                                googleAuthR_2.0.0                           munsell_0.5.0                              
[193] e1071_1.7-12                                GenomeInfoDbData_1.2.9                      reshape2_1.4.4                             
[196] gtable_0.3.1                               
>

JonnyBaseball avatar Dec 03 '22 20:12 JonnyBaseball

BUG DESCRIPTION (SUPPLEMENT TO ISSUE)

I was also unable to install Polyfun module with your example data and here I responded 'y' to prompt to install:

REPRODUCIBLE EXAMPLE

topSNPs <- echodata::import_topSNPs(

  • topSS = echodata::topSNPs_Nalls2019_raw,
  • colmap = colmap,
  • grouping_vars = "Locus Number")
  • Constructing locus names from CHR and index SNP. Standardising column headers. First line of summary statistics file: SNP CHR BP Nearest Gene QTL Nominated Gene (nearest QTL) Effect allele Other allele Effect allele frequency Beta, all studies SE, all studies P, all studies P, COJO, all studies P, random effects, all studies P, Conditional 23AndMe only P, 23AndMe only I2, all studies Freq1, previous studies Beta, previous studies StdErr, previous studies P, previous studies I2, previous studies Freq1, new studies Beta, new studies StdErr, new studies P, new studies I2, new studies Passes pooled 23andMe QC Known GWAS locus within 1MB Failed final filtering and QC Locus within 250KB Locus Number Locus Gene Returning unmapped column names without making them uppercase.
  • Mapping colnames from MungeSumstats ==> echolocatoR

fullSS_path <- echodata::example_fullSS(dataset = "Nalls2019") Writing file to ==> /var/folders/rl/6vqvm_h5319fml5s4vw8fbfr0000gn/T//RtmpNSorp8/nalls2019.fullSS_subset.tsv

results <- echolocatoR::finemap_loci(

  • fullSS_path = fullSS_path,
  • topSNPs = topSNPs,
  • loci = c("BST1"),
  • LD_reference = "1KGphase3",
  • #dataset_name = "Nalls23andMe_2019",
  • fullSS_genome_build = "hg19",
  • bp_distance = 1000,
  • finemap_methods = c("ABF","SUSIE","FINEMAP","POLYFUN_FINEMAP","POLYFUN_SUSIE","PAINTOR","COJO_conditional","COJO_joint","COJO_stepwise"),
  • munged = TRUE)

CONSOLE OUTPUT

PolyFun is not installed. Reinstall echofinemap with PolyFun? This will automatically restart R. (y/n): y Installing PolyFun submodule. Cloning into '/var/folders/rl/6vqvm_h5319fml5s4vw8fbfr0000gn/T//RtmpNSorp8/file9c3057a3dc73'... fatal: unable to access 'https://github.com/RajLabMSSM/echofinemap/': Could not resolve host: github.com Error in package_file(): ! '/var/folders/rl/6vqvm_h5319fml5s4vw8fbfr0000gn/T//RtmpNSorp8/file9c3057a3dc73' is not a directory. Run rlang::last_error() to see where the error occurred.

rlang::last_error() <error/rlang_error> Error in package_file(): ! '/var/folders/rl/6vqvm_h5319fml5s4vw8fbfr0000gn/T//RtmpNSorp8/file9c3057a3dc73' is not a directory.

Backtrace:

  1. echolocatoR::finemap_loci(...)
  2. echofinemap::POLYFUN_install()
  3. echofinemap:::install_submodule_git("https://github.com/RajLabMSSM/echofinemap")
  4. devtools::install(install_dir, ...)
  5. devtools::as.package(pkg)
  6. devtools::package_file(path = x) Run rlang::last_trace() to see the full context.
BUG DESCRIPTION As well, I was unable to access PAINTOR

REPRODUCIBLE EXAMPLE

results <- echolocatoR::finemap_loci(

  • fullSS_path = fullSS_path,
  • topSNPs = topSNPs,
  • loci = c("BST1"),
  • LD_reference = "1KGphase3",
  • #dataset_name = "Nalls23andMe_2019",
  • fullSS_genome_build = "hg19",
  • bp_distance = 1000,
  • finemap_methods = c("ABF","SUSIE","FINEMAP","PAINTOR","COJO_conditional","COJO_joint","COJO_stepwise"),
  • munged = TRUE)

CONSOLE OUTPUT

Error in PAINTOR_find_executable(paintor_path = paintor_path) : PAINTOR executable cannot be found.

SESSION INFO

utils::sessionInfo() R version 4.2.0 (2022-04-22) Platform: x86_64-apple-darwin17.0 (64-bit) Running under: macOS Catalina 10.15.7

Matrix products: default BLAS: /Library/Frameworks/R.framework/Versions/4.2/Resources/lib/libRblas.0.dylib LAPACK: /Library/Frameworks/R.framework/Versions/4.2/Resources/lib/libRlapack.dylib

locale: [1] en_US.UTF-8/en_US.UTF-8/en_US.UTF-8/C/en_US.UTF-8/en_US.UTF-8

attached base packages: [1] stats4 stats graphics grDevices utils datasets methods base

other attached packages: [1] forestploter_0.2.3 snpStats_1.48.0 Matrix_1.5-3 survival_3.4-0 readr_2.1.3 GenomeInfoDb_1.34.3 IRanges_2.32.0
[8] S4Vectors_0.36.0 BiocGenerics_0.44.0 echolocatoR_2.0.3

loaded via a namespace (and not attached): [1] rappdirs_0.3.3 rtracklayer_1.58.0 GGally_2.1.2
[4] R.methodsS3_1.8.2 ragg_1.2.4 tidyr_1.2.1
[7] echoLD_0.99.8 ggplot2_3.4.0 bit64_4.0.5
[10] knitr_1.41 irlba_2.3.5.1 DelayedArray_0.24.0
[13] R.utils_2.12.2 data.table_1.14.6 rpart_4.1.19
[16] KEGGREST_1.38.0 RCurl_1.98-1.9 AnnotationFilter_1.22.0
[19] generics_0.1.3 GenomicFeatures_1.50.2 callr_3.7.3
[22] usethis_2.1.6 RSQLite_2.2.19 proxy_0.4-27
[25] bit_4.0.5 tzdb_0.3.0 httpuv_1.6.6
[28] xml2_1.3.3 SummarizedExperiment_1.28.0 assertthat_0.2.1
[31] viridis_0.6.2 gargle_1.2.1 xfun_0.35
[34] hms_1.1.2 promises_1.2.0.1 fansi_1.0.3
[37] restfulr_0.0.15 progress_1.2.2 dbplyr_2.2.1
[40] readxl_1.4.1 Rgraphviz_2.42.0 igraph_1.3.5
[43] DBI_1.1.3 htmlwidgets_1.5.4 reshape_0.8.9
[46] downloadR_0.99.5 purrr_0.3.5 ellipsis_0.3.2
[49] dplyr_1.0.10 ggnewscale_0.4.8 backports_1.4.1
[52] biomaRt_2.54.0 deldir_1.0-6 MatrixGenerics_1.10.0
[55] MungeSumstats_1.7.2 vctrs_0.5.1 Biobase_2.58.0
[58] remotes_2.4.2 ensembldb_2.22.0 cachem_1.0.6
[61] withr_2.5.0 BSgenome_1.66.1 checkmate_2.1.0
[64] vroom_1.6.0 GenomicAlignments_1.34.0 prettyunits_1.1.1
[67] cluster_2.1.4 ape_5.6-2 dir.expiry_1.6.0
[70] lazyeval_0.2.2 crayon_1.5.2 basilisk.utils_1.10.0
[73] crul_1.3 pkgconfig_2.0.3 labeling_0.4.2
[76] pkgload_1.3.2 nlme_3.1-160 ProtGenerics_1.30.0
[79] XGR_1.1.8 devtools_2.4.5 nnet_7.3-18
[82] pals_1.7 rlang_1.0.6 miniUI_0.1.1.1
[85] lifecycle_1.0.3 filelock_1.0.2 httpcode_0.3.0
[88] BiocFileCache_2.6.0 echotabix_0.99.8 dichromat_2.0-0.1
[91] cellranger_1.1.0 coloc_5.1.0.1 matrixStats_0.63.0
[94] graph_1.76.0 osfr_0.2.9 boot_1.3-28.1
[97] base64enc_0.1-3 processx_3.8.0 png_0.1-8
[100] viridisLite_0.4.1 rjson_0.2.21 rootSolve_1.8.2.3
[103] bitops_1.0-7 R.oo_1.25.0 ggnetwork_0.5.10
[106] Biostrings_2.66.0 blob_1.2.3 mixsqp_0.3-48
[109] stringr_1.4.1 echoplot_0.99.6 dnet_1.1.7
[112] jpeg_0.1-9 echodata_0.99.16 BSgenome.Hsapiens.1000genomes.hs37d5_0.99.1 [115] scales_1.2.1 memoise_2.0.1 magrittr_2.0.3
[118] plyr_1.8.8 hexbin_1.28.2 zlibbioc_1.44.0
[121] compiler_4.2.0 echoconda_0.99.8 BiocIO_1.8.0
[124] RColorBrewer_1.1-3 catalogueR_1.0.0 EnsDb.Hsapiens.v75_2.99.0
[127] Rsamtools_2.14.0 cli_3.4.1 urlchecker_1.0.1
[130] XVector_0.38.0 echoannot_0.99.10 ps_1.7.2
[133] patchwork_1.1.2 htmlTable_2.4.1 Formula_1.2-4
[136] MASS_7.3-58.1 tidyselect_1.2.0 stringi_1.7.8
[139] textshaping_0.3.6 yaml_2.3.6 supraHex_1.36.0
[142] latticeExtra_0.6-30 ggrepel_0.9.2 grid_4.2.0
[145] VariantAnnotation_1.44.0 tools_4.2.0 lmom_2.9
[148] parallel_4.2.0 rstudioapi_0.14 foreign_0.8-83
[151] piggyback_0.1.4 gridExtra_2.3 gld_2.6.6
[154] farver_2.1.1 RcppZiggurat_0.1.6 digest_0.6.30
[157] BiocManager_1.30.19 shiny_1.7.3 Rcpp_1.0.9
[160] GenomicRanges_1.50.1 later_1.3.0 OrganismDbi_1.40.0
[163] httr_1.4.4 AnnotationDbi_1.60.0 RCircos_1.2.2
[166] ggbio_1.46.0 biovizBase_1.46.0 colorspace_2.0-3
[169] XML_3.99-0.12 fs_1.5.2 reticulate_1.26
[172] splines_4.2.0 RBGL_1.74.0 expm_0.999-6
[175] echofinemap_0.99.4 basilisk_1.10.2 Exact_3.2
[178] mapproj_1.2.9 SNPlocs.Hsapiens.dbSNP155.GRCh37_0.99.22 sessioninfo_1.2.2
[181] systemfonts_1.0.4 xtable_1.8-4 jsonlite_1.8.3
[184] Rfast_2.0.6 susieR_0.12.27 R6_2.5.1
[187] profvis_0.3.7 Hmisc_4.7-2 mime_0.12
[190] pillar_1.8.1 htmltools_0.5.3 glue_1.6.2
[193] fastmap_1.1.0 DT_0.26 BiocParallel_1.32.3
[196] class_7.3-20 codetools_0.2-18 maps_3.4.1
[199] pkgbuild_1.4.0 mvtnorm_1.1-3 utf8_1.2.2
[202] lattice_0.20-45 tibble_3.1.8 curl_4.3.3
[205] DescTools_0.99.47 zip_2.2.2 openxlsx_4.2.5.1
[208] interp_1.1-3 googleAuthR_2.0.0 munsell_0.5.0
[211] e1071_1.7-12 GenomeInfoDbData_1.2.9 reshape2_1.4.4
[214] gtable_0.3.1
2022-12-06 12:40:35.793 R[39984:29884093] -deltaZ is deprecated for NSEventTypeMagnify. Please use -magnification.

JonnyBaseball avatar Dec 06 '22 17:12 JonnyBaseball

I tried again after reinstalling echolocatoR - with your example - got farther than before - but with final line:

Error in echofinemap::POLYFUN_install() : PolyFun is not installed. Resources/bin/R CMD INSTALL \

REPRODUCIBLE EXAMPLE

results <- echolocatoR::finemap_loci(

  • fullSS_path = fullSS_path,
  • topSNPs = topSNPs,
  • loci = c("BST1"),
  • LD_reference = "1KGphase3",
  • #dataset_name = "Nalls23andMe_2019",
  • fullSS_genome_build = "hg19",
  • bp_distance = 1000,
  • finemap_methods = c("ABF","SUSIE","FINEMAP","POLYFUN_SUSIE","POLYFUN_FINEMAP"),
  • munged = TRUE)

CONSOLE OUTPUT

PolyFun is not installed. Reinstall echofinemap with PolyFun? This will automatically restart R. (y/n): Y Installing PolyFun submodule. Cloning into '/var/folders/rl/6vqvm_h5319fml5s4vw8fbfr0000gn/T//Rtmp7yb4YZ/filef76f4b24f0c'... Submodule 'inst/tools/PAINTOR_V3.0' (https://github.com/gkichaev/PAINTOR_V3.0.git) registered for path 'inst/tools/PAINTOR_V3.0' Submodule 'inst/tools/polyfun' (https://github.com/omerwe/polyfun.git/) registered for path 'inst/tools/polyfun' Cloning into '/private/var/folders/rl/6vqvm_h5319fml5s4vw8fbfr0000gn/T/Rtmp7yb4YZ/filef76f4b24f0c/inst/tools/PAINTOR_V3.0'... Cloning into '/private/var/folders/rl/6vqvm_h5319fml5s4vw8fbfr0000gn/T/Rtmp7yb4YZ/filef76f4b24f0c/inst/tools/polyfun'... Submodule path 'inst/tools/PAINTOR_V3.0': checked out 'ffce82a7114eeb98220db39ba5d6b69e5a419a9e' Submodule path 'inst/tools/polyfun': checked out 'a9a2274906ef0737a48c909ba0c86d03272a4833' ── R CMD build ────────────────────────────────────────────────────────────────────────────────────────────────────────── ✔ checking for file ‘/private/var/folders/rl/6vqvm_h5319fml5s4vw8fbfr0000gn/T/Rtmp7yb4YZ/filef76f4b24f0c/DESCRIPTION’ ─ preparing ‘echofinemap’: (986ms)folders/rl/6vqvm_h5319fml5s4vw8fbfr0000gn/T/Rtmp7yb4YZ/filef76f4b24f0c/DESCRIPTION’ ─ checking for LF line-endings in source and make files and shell scripts (358ms) Running /Library/Frameworks/R.framework/Resources/bin/R CMD INSTALL
/var/folders/rl/6vqvm_h5319fml5s4vw8fbfr0000gn/T//Rtmp7yb4YZ/echofinemap_0.99.4.tar.gz --install-tests

  • installing to library ‘/Library/Frameworks/R.framework/Versions/4.2/Resources/library’
  • installing source package ‘echofinemap’ ... ** using staged installation ** R ** inst ** tests ** byte-compile and prepare package for lazy loading ** help *** installing help indices ** building package indices ** installing vignettes ** testing if installed package can be loaded from temporary location ** testing if installed package can be loaded from final location ** testing if installed package keeps a record of temporary installation path
  • DONE (echofinemap) Error in echofinemap::POLYFUN_install() : PolyFun is not installed. Resources/bin/R CMD INSTALL \

JonnyBaseball avatar Dec 07 '22 21:12 JonnyBaseball

Same here! Do you perhaps have a solution now?

laleoarrow avatar Nov 22 '24 13:11 laleoarrow