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--cut_right not working as expected

Open bnwlf opened this issue 4 years ago • 0 comments

Hi guys,

I used the following command to call fastp:

fastp -i reads/XXXXXXXXXXX_S81_L001_R1_001.fastq.gz -I reads/XXXXXXXXXXX_S81_L001_R2_001.fastq.gz -o R1.fastq.gz -O R2.fastq.gz --cut_right -M 30 --cut_right_window_size 1 -w 10

I would expect that every basecall with a phred blow 30 and every later basecall is removed by fastp.

If I analyze the output using fastQC I got such an unexpected behavior:

image

For me it looks like the last basecall of a reads is ignored by the sliding window by an off by one error.

Installed fastp via conda (v0.20.1)

Cheers! Ben

bnwlf avatar Jun 08 '21 11:06 bnwlf