Guppy software version for base calling

[mmiladi]

Hello,

Thanks for providing this tool! I was wondering if the version of Guppy can have an impact on the prediction quality and performance. I have data called using Guppy 4.0.9 and 3.2.10. Do they expect to work with the latest version of xpore? Would you recommend to re-base call them with a specific version of Guppy to get a reliable performance of m6A predictions?

Best, Milad

[ploy-np]

Hi @mmiladi,

Sorry for the delayed response. In theory, it shouldn't make much different. In the paper, different Guppy versions were used. However, I recommend to use the same Guppy version across all samples if possible.