Inquiry about the result interpretation and m6A level

[kwonej0617]

Hi @yuukiiwa

I wonder if there's a way to get m6A level, which can be defined by the number of m6a-modifying copies out of total number of copies of each transcript?

In addition, could you please explain how you define mod_rate? I read the paper but it is quite hard to understand the concept.

Thank you!

[yuukiiwa]

Hi @kwonej0617,

xpore estimates the unmodified and modified distributions. A low mod_rate is reported if more current signals of a site are under the unmodified distribution than the modified distribution while a high mod rate is reported if more current signals of a site are under the modified distribution than the unmodified distribution.

xpore does not output read-level outputs, so we recommend using m6anet instead to obtain the proportion of m6A-modified reads in a site. You can combine the results from m6anet and xpore to select the m6A sites that are differentially modified in the different conditions.

Thanks!

Best wishes, Yuk Kei