Visual Behavior paper controls

[yavorska-iryna]

• Read Kyle's paper on modeling omission responses
• shuffle control of all Cre lines clusters
• area and depth analysis of clusters
• selecting number of k (14 with a few clusters that get dropped due to size)

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• Behavior cluster cells, are some more running vs pupil encoding?
• What can we say about task coding cluster? Examine the kernels for each group of coding.

[yavorska-iryna]

11.13.23

• proposed area and depth figure

• debugging multi session df notebook to load cell response metrics

[yavorska-iryna]

23.11.30

A few observations about distributions of response metrics for different clusters

Cluster 2 Novel and Cluster 4 Familiar: (Are responses to Novel stimuli different than responses to Familiar stimuli? Yes)

• Novel responses are more reliable and have higher image tuning than Familiar responses
• Novel responses are larger means than Familiar response (to preferred image)
• Running modulation of responses to all Novel stimuli are enhancing, while responses to familiar stimuli also have suppressed by running population. This is specific to all images, not preferred image and only for Excitatory cells.

Cluster 2 Novel and Cluster 3 Novel: (Are responses of cells that are experience selective different from responses of cells that are Novel image preferring? Yes)

• Both clusters have similar reliability and running modulation.
• Cluster 2 has higher image selectivity, fano factor, and mean responses than Cluster 3.

Cluster 3 Novel and Novel +: (Are responses metrics of the same cells to Novel stimuli change over time? No)

• Overall, responses to all images are very similar, as is running modulation and image selectivity.

**Cluster 3 Novel + and Cluster 4 Familiar: (How do Novel+ responses compare to familiar responses?)

• Novel+ responses have higher fano factor than Familiar responses, but reliability is the same.
• Image selectivity is the same (This is surprising, double check)
• running modulation is the same (it appears that Novel+ responses start getting suppressed by running)

Cluster 4 Familiar and Cluster 5 Novel+: (How do cells that sponteneously respond to Novel+ images differ from Familiar responses?)

• spontaneous Novel+ responses have higher mean responses and higher fano factor than Familiar responses
• image selectivity and running modulation are the same

Cluster 4 Familiar and Cluster 7 Familiar: (Do the responses to Familiar images differ between cells that only encode Familiar vs those that also code for Novelty? Yes!)

• Familiar responses in cluster 7 look more like Novel responses in cluster 2! They have higher fano factor, image selectivity and lack suppression by running!
• Cluster 7 familiar responses also have higher mean responses.

Cluster 7 Familiar and Novel: (So, cells that respond to Familiar images similarly to cells that have Novelty responses, are their responses to Novelty also the same? yes and no)

• Seems like cluster 7 responses to Familiar images are more selective than Novel images
• Differences in reliability, fano factor and modulation seem to be preferred image specific, while mean responses to preferred stimuli are modestly higher to Familiar images.

Cluster 6 Familiar and Novel: (Do cells that code for images regardless of experience level show changes in response properties? Not really)

• There are some modest differences, p values will be higher with within the same sample test but not sure if would withstand multiple correction